Globularia Alypum L. Modulates Inflammatory Markers in the Human Colon and shows a Potential Antioxidant Role in Myeloid Leukemic Cells

Najla Hajji, Department of Medicine, Surgery and Dentistry, Scuola Medica Salernitana, University of Salerno, Fisciano (SA) Italy
Elodie Hudik, Université Paris-Sud and Université Paris-Saclay, Orsay, France; CNRS, LCP, and INSERM U1174, Orsay, France
Paola Iovino, Department of Medicine, Surgery and Dentistry, Scuola Medica Salernitana, University of Salerno, Fisciano (SA) Italy
Nacim Zouari, Higher Institute of Applied Biology of Medenine, Medenine, Gabes University, Tunisia
Hichem Sebai, Laboratoire de Physiologie Fonctionnelle et Valorisation des Bio-Ressources - Institut Supérieur de Biotechnologie de Béja, Université de Jendouba, Avenue Habib Bourguiba, Béja, Tunisia
Oliver Nusse, Université Paris-Sud and Université Paris-Saclay, Orsay, France; CNRS, LCP, and INSERM U1174, Orsay, France
Carolina Ciacci, Department of Medicine, Surgery and Dentistry, Scuola Medica Salernitana, University of Salerno, Fisciano (SA) Italy

Abstract

Globularia alypum (GA), a plant of the Globulariacea family, has long been used as a traditional cure for inflammatory and metabolic illnesses.In addition to various in vitro model studies, the current work focuses on GA'santioxidant and anti-inflammatory properties in human colon biopsies.

Liquid Chromatography-Electrospray Ionization Mass Spectrometry identified the phenol components in GA aqueous extract (GAAE).The antioxidant ability of GAAE was tested in vitro utilizing chemiluminescence and flow cytometry using fluorescent yeastsn conjunction with PLB-985-human myeloid leukemia cells. Experiments on human colon biopsies after a biopsy challenge with Escherichia coli-lipopolysaccharides aimed tosee if GAAE had an anti-inflammatory impact on human colon inflammation.Western blotting was used to assessthe expression of several inflammatory markers.

According to the findings, GAAE had a significant influence on hydrogen peroxide and cellular reactive oxygen species.GAAE inhibited the activities of cyclooxygenase 2 and nuclear factor B in inflamed biopsies, indicating anti-inflammatory action.The present study is the first to show that GA has a beneficial effect on human colon inflammation, thanks to its significant antioxidant activity in vitro.According to these preliminary data, GA should be further studies integrative supporting human gastrointestinal inflammatory diseases.

Recommended Citation

Hajji, Najla; Hudik, Elodie; Iovino, Paola; Zouari, Nacim; Sebai, Hichem; Nusse, Oliver; and Ciacci, Carolina (2021) "Globularia Alypum L. Modulates Inflammatory Markers in the Human Colon and shows a Potential Antioxidant Role in Myeloid Leukemic Cells," Translational Medicine @ UniSa: Vol. 24 : Iss. 1 , Article 4.
Available at: https://doi.org/10.37825/2239-9747.1028